Japanese | English

トップページへ戻る

詳細

   
フルテキストURLフルテキストリンクなし
タイトル螢光顕微鏡によるラットの組織内のビタミンD_3の分布の観察
タイトルヨミケイコウケンビキョウ ニヨル ラット ノ ソシキナイ ノ ビタミン D 3 ノ ブンプ ノ カンサツ
タイトル別表記A Fluorescence Microscopic Observation of Vitamin D_3 Distribution in the Tissue of the Rat.
作成者牧田, 登之
松浦, 博義
木脇, 祐順
作成者ヨミマキタ, タカシ
マツウラ, ヒロヨシ
キワキ, スケチカ
作成者別表記Makita, Takashi
Matsuura, Hiroyoshi
Kiwaki, Sukechika
作成者所属山口大学農学部
内容記述(抄録等)It has been reported that the oral administration of vitamin D_2 dissolved in olive oil followed by formaldehyde fixation reveals vitamin D_2 as yellowish spots under the fluorescence microscope. In order to compare this technic with the electron microscopic investigation of the effect of vitamin D_3 on the alkaline- and adenosine- triphosphatase activity in the intestine, the followinf modifications were tried: (i) repeated intramuscular injections of vitamin D_3 solution instead of a single oral administraion of vitamin D_2 dissolved in oil, and (ii) glutaraldehyde fixation instead of paraformaldehyde fixation. The studies were performed in normal adult rats. Intramuscular injevtions of aqueous solution of vitamin D_3 resulted in the same distribution of fluorescent spots as those obtained by the oral administration. Repeated injection of fluorescent spots as those obtained by the oral administration. Repeated injections (10,000 I. U. to 130,000 I. U.) slightly enhanced the fluorescence but revealed the same localization as that with a single dose. The fluorescence with glutaraldehyde was similar to that seen with paraformaldehyde. The interval between the last injection and the examination appeared to be the most important determinant in the histological localization of the fluorescence. ...
本文言語jpn
主題農学
資料タイプtext
出版者山口大学農学部
出版者ヨミヤマグチ ダイガク ノウガクブ
NII資料タイプ紀要論文
ISSN0513-1715
NCIDAN00244250
学内刊行物(紀要等)山口大学農学部学術報告
掲載誌名山口大学農学部学術報告
掲載誌名別表記Bulletin of the Faculty of Agriculture, Yamaguchi University
23
開始ページ515
終了ページ530
発行日1972
著者版/出版社版その他
リポジトリIDD490023000006
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/D490023000006