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タイトル日本脳炎ウイルスの培養細胞内増殖に関する研究
タイトルヨミニホンノウエン ウイルス ノ バイヨウサイボウナイゾウショク ニ カン スル ケンキュウ
タイトル別表記Growth of Japanese encephalitis virus in tissue cultures
作成者冨田, 正章
戸田, 光敬
鹿江, 雅光
作成者ヨミトミタ, マサアキ
トダ, ミツヨシ
カノエ, マサミツ
作成者別表記Tomita, Masaaki
Toda, Mitsuyoshi
Kanoe, Masamitsu
作成者所属山口大学農学部
内容記述(抄録等)The development of Japanese encephalitis virus (JaGAr 01 strain) in the cell cultures was investigated by the methods of fluorescent antibody technic and electron microscopy. The results obtained were as follows: 1) Cytopathic effect after virus inoculation was observed in BHK-21 and HK cells at 48 hr, and Vero and HmLu cells at 72 hr, respectively. The highest titers of infectivity in the media were demonstrated at 46 to 96 hr after virus inoculation and significant correlation was ovserved between infective titers and HA. 2) In infective cell cultures, viral specific fluorescences were observed in BHK-21 cells at 10 hr, in HmLu cells at 14 hr, in Vero and HK cells at 16 hr, and in CE cells at 18 hr, respectively. At initial stage of infection, the fluorescence appeared around the perinuclear area of a single cell and developed into cytoplasmic area with granular or radial form. These cells formed gradually into a mass. At 24 hr after virus inoculation, a mass of 10 to 20 cells was predominantly observed. 3) In electron microscopy, viral particle was approximately 40nm in diameter. It was composed of an outer membrane, viroplasm, and an electron dense uncleoid which was 25 nm in diameter. Viral particles, isolated or clustered, were observed at extracellular space near the cell membrane, in intracellular vacuole, in phagosome, and in enlarged endoplasmic reticulum fulled with granular matrix, but not in nucleus.
本文言語jpn
主題農学
資料タイプtext
出版者山口大学農学部
出版者ヨミヤマグチ ダイガク ノウガクブ
NII資料タイプ紀要論文
ISSN0513-1715
NCIDAN00244250
学内刊行物(紀要等)山口大学農学部学術報告
掲載誌名山口大学農学部学術報告
掲載誌名別表記Bulletin of the Faculty of Agriculture, Yamaguchi University
29
開始ページ31
終了ページ46
発行日1978
著者版/出版社版その他
リポジトリIDD490029000004
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/D490029000004