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フルテキストURLKJ00000156727.pdf ( 496.4KB ) 公開日 2010-04-19
タイトル培養脳細胞の全膜電位固定法, 及び局所的膜電位固定法によるグルタメイトチャネル特性の解析
タイトルヨミバイヨウ ノウサイボウ ノ ゼン マクデンイ コテイホウ オヨビ キョクショテキ マクデンイ コテイホウ ニヨル グルタメイト チャネル トクセイ ノ カイセキ
タイトル別表記Analyses of Glutamate Current Fluctuations and Glutamate Single Channel Currents of Cultured Brain Neurons
作成者永岡, 諭
蛯名, 良雄
新貝, 鉚蔵
橋本, 基
作成者ヨミナガオカ, サトシ
エビナ, ヨシオ
シンガイ, リュウゾウ
ハシモト, ハジメ
作成者別表記Nagaoka, Satoshi
Ebina, Yoshio
Shingai, Ryuzo
Hashimoto, Hajime
作成者所属山口大学工学部
内容記述(抄録等)Glutamate is an excitatory neurotransmitter which activates glutamate ion channels located on the membrane of neurons. We have investigated kinetics of the ion channels activated by application of glutamate or its agonist N-methyl-D-asparate (NMDA) in cultured rat brain neurons. Methods of recording from the neurons are the whole cell clamp to make clear characteristics of the glutamate channels in the whole cell by analyzing the membrane current fluctuation, and the patch clamp to record directly the gatings of one or a few channels and to study their statistical characteristics in detail. Power spectrum density (PWS) of current fluctuations is obtained by FFT. The mean open time and the mean conductance of the channels are estimated by fitting PWS to the theoretical PWS curve (Lorentzian) by computer calculations or eye inspection. PWS fits, in most cases, a sum of two Lorentzian functions for glutamate current, whereas that of NMDA current fits a single Lorentzian with few exceptions. The fluctuated currents are depressed by Mg ions in the saline in negative membrane potential region. Fluctuation analysis shows that the opening probability decreases by the presence of Mg ions. The histograms of the conductans and the open time of NMDA channel obtained from patch clamp data. The mean open time estimated from the FFT anaiysis is 6 msec, which is close to the value (9 msec) estimated from the assumption that the mean open time obtained from the patch clamp recording has an exponential distribution.
本文言語jpn
主題電気電子工学
資料タイプtext
ファイル形式application/pdf
出版者山口大学工学部
出版者ヨミヤマグチ ダイガク コウガクブ
NII資料タイプ紀要論文
ISSN0372-7661
NCIDAN00244228
学内刊行物(紀要等)山口大学工学部研究報告
掲載誌名山口大学工学部研究報告
掲載誌名別表記Memoirs of the Faculty of Engineering, Yamaguchi University
39
1
開始ページ251
終了ページ257
発行日1988-10
著者版/出版社版出版社版
備考本文データは国立情報学研究所において電子化したものである
リポジトリIDKJ00000156727
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/KJ00000156727