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タイトル多形性膠芽腫T98G細胞における化学療法のProliferating cell nuclear antigenに及ぼす影響について : ranimustineとetoposideを用いた検討
タイトルヨミタケイセイ コウガシュ T98G サイボウ ニオケル カガク リョウホウ ノ Proliferating cell nuclear antigen ニ オヨボス エイキョウ ニツイテ : ranimustine ト etoposide オ モチイタ ケントウ
タイトル別表記The Influence of Chemotherapy on Proliferating Cell Nuclear Antigen of Glioblastoma Cell Line
作成者古谷, 康浩
作成者ヨミフルタニ, ヤスヒロ
作成者別表記Furutani,Yasuhiro
作成者所属山口大学医学部
内容記述(抄録等)In this study we analized cell kinetics of glioblastoma cell line (T98G) under growth suppresive state by use of PCNA-DNA and BrdU-DNA simultaneous double stain method (flow cytometry). The growth suppresive state was induced by two kinds of anticancer agents of MCNU and etoposide. In spite of decrease of BrdU antigen by both anticancer agents, PCNA antigen and PCNA positive ratio increased compared with that of control in the early stage of chemotherapy. Especially PCNA antigen was increased more than two times of control by the proliferation suppresive concentration of etoposide that acted after consumption of PCNA in the series of DNA synthesis. We considered that was caused by change of cell cycle, negative feedback against growth suppresive state and suppression of comsumption of PCNA. It can be summarized that PCNA antigen and PCNA positive ratio under growth suppresive state by anticancer agent don't reflect truly tumor proliferation in the early stage of chemotherapy, and we can't judge the effect of anticancer agent by analysis of cell kinetics used PCNA. In the future, compared with control growth suppresive state would be caught in early stage of chemotherapy by increase of PCNA antigen.
本文言語jpn
著者キーワードglioblastoma cell line
chemotherapy
proliferating cell nuclear antigen
主題医学
資料タイプtext
出版者山口大学医学会
出版者ヨミヤマグチ ダイガク イガッカイ
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN0513-1731
NCIDAN00243156
学内刊行物(紀要等)山口医学
掲載誌名山口医学
43
1
開始ページ79
終了ページ86
発行日1994-02
著者版/出版社版その他
リポジトリIDB030043000110
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/B030043000110