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タイトルプラスチックストロー内に封入された卵子の位置が凍結過程で透明帯と細胞に与える影響
タイトルヨミプラスチック ストロー ナイ ニ フウニュウ サレタ ランシ ノ イチ ガ トウケツ カテイ デ トウメイタイ ト サイボウ ニ アタエル エイキョウ
タイトル別表記Deterimental Effects of Placement for Deep Freezing against Zonae Pellucida and Cell of Bovine Oocytes Filled in Plastic Straws
作成者鈴木, 達行
林, 司
作成者ヨミスズキ, タツユキ
ハヤシ, ツカサ
作成者別表記Suzuki, Tatsuyuki
Hayashi, Tsukasa
作成者所属山口大学農学部
内容記述(抄録等)The deleterious effects against zonae pellucida and cell bovine oocytes filled into plastic straws frozen at the vertical or horizontal settings were compared. In the first experiment 1.320 bovine oocytes were suspended in 1.4M glycerol in Phosphate buffered saline (PBS), and in the second one 206 bovine oocytes were suspended in 1.4M glycerol and 0.3M sucrose in PBS. After the equilibration in 1.4M glycerol in PBS for 10 minutes, each plastic straw filled with the bovine oocyte was kept deep directly in an alcohol bath at -6℃ at the vertical or horizontal settings for 10 minutes. Thereafter, The straws were gradually cooled to -30℃ at a cooling rate of 0.3℃/min before stored in liquid nitrogen. To evaluate the morphological deformations incurred during the above manipulations each sample of frozen oocytes was thawed just before microscopic examinations at 100-200 fold magnification. Lost rates of oocytes frozen at the vertical and horizontal settins were 2% (13/628) and 0.4% (3/692) respectively. When straws were frozen at the vertical setting, the incidence of deformed zonae pellucida and cell was 31.1% (191/615) and 18% (111/615), and those were significantly higher (p<0.05) than 22.8% (157/689) and 10% (69/689) obtained with the straws frozen at the horizontal setting. However, no significant differences were discernible in the incidence of deformed oocytes between the vertical setting (5.8% ; 6/104) and the horizontal setting (6.9% ; 8/116), using different suspending medium 1.4M glycerol and 0.3M sucrose in PBS.
本文言語jpn
著者キーワードFrozen oocytes
straw setting
damage
zonae pellucida
主題農学
資料タイプtext
出版者山口大学農学部
出版者ヨミヤマグチ ダイガク ノウガクブ
NII資料タイプ紀要論文
ISSN0513-1715
NCIDAN00244250
学内刊行物(紀要等)山口大学農学部学術報告
掲載誌名山口大学農学部学術報告
掲載誌名別表記Bulletin of the Faculty of Agriculture, Yamaguchi University
39
開始ページ1
終了ページ6
発行日1991-12
著者版/出版社版その他
リポジトリIDD490039000001
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/D490039000001