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タイトルニワトリとウズラの艀化前後の腸管のアルカリ性ホスファターゼ活性の変動 : I.生化学的測定
タイトルヨミニワトリ ト ウズラ ノ フカゼンゴ ノ チョウカン ノ アルカリ セイ ホスファターゼ カッセイ ノ ヘンドウ : Iセイカガクテキソクテイ
タイトル別表記Pre- and Post-hatching Development of the ALP-ase Activity in the Small Intestine of the Chick and Quail. : I. Biochemical Evaluation.
作成者牧田, 登之
香川, 和三
原, 行雄
木脇, 祐順
作成者ヨミマキタ, タカシ
カガワ, カズミツ
ハラ, ユキオ
作成者別表記Makita, Takashi
Kagawa, Kazumitu
Hara, Yukio
Kiwaki, Sukechika
作成者所属山口大学農学部
内容記述(抄録等)In order to correlate the biochemical determination of the phosphatase activity to its histochemical and cytochemical localization the alkaline phosphatase activity in the small intestine of pre and post hatch chick and quail was estimated by colorimetry. Using phenylphosphate as substrate the supernatant of homogenate of three segments, duodenum, jejunum and ileum from each group of embryo or chick was incubated for 15 min. at lit and optical density was read in a Klett -Summerson spectrophotometer. The result obtained was then transformed into King-Armstrong units. At pre-hatching stages the ALP-ase activity in every semgents of intestine was considerably lower than that had been implied by histochemical works. It was estimated that the activity detectable by histochemical studies using /3 glycerophosphate as substrate might be comparable to those higher than 10 King-Armstrong unit in this study. Actually the activity until one or two days before hatch remainded lower than 10 units. The ALP ase activity then drastically increased during the period from the last day of the incubation to the first or second day of the post-hatch life. When maximum activity was attained it ranged from 115 to 160 in the duodenum, 65 to 100 in the jejunum and 35 to 45 in the ileum. Shortly after this upserge the ALP-ase activity sharply fell to the level of that of two months old chick. The average activity from the duodenum, jejunum, and ileum of the two months old chicks was 87ア10, 42ア 4 , and 8 ア 3 , respectively. A proximodistal gradient of the ALP-ase activity in the small intestine was suggested by the highest activity in the duodenum, the moderate one in the jejunum and the least in the ileum. (Received June 30, 1974)
本文言語jpn
主題農学
資料タイプtext
出版者山口大学農学部
出版者ヨミヤマグチ ダイガク ノウガクブ
NII資料タイプ紀要論文
ISSN0513-1715
NCIDAN00244250
学内刊行物(紀要等)山口大学農学部学術報告
掲載誌名山口大学農学部学術報告
掲載誌名別表記Bulletin of the Faculty of Agriculture, Yamaguchi University
25
開始ページ1029
終了ページ1040
発行日1974
著者版/出版社版その他
リポジトリIDD490025000011
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/D490025000011