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フルテキストURLA020049000305.pdf ( 292.7KB ) 公開日 2010-04-19
タイトルImpact of Hyperosmotic Glycerol on Tighy Junction In Vitro
作成者Tominaga, Takashi
作成者ヨミトミナガ, タカシ
作成者所属山口大学医学部
内容記述(抄録等)The tight junction regulates passage of molecules throuth the paracellular spaces. Occludin and claudins are the specific trancmembrance protains present at the tight junction and are believed to regulate the cell barrier functions. To examine the response of the tight junction to hyperosmotic solutions, Ⅰinvestigated the effects of hyperosmotic glycerol on function and protein expression of the tight junction in ECV304 cells. Cell cytotoxicity analysis showed that the high (10%) concentration of glcerol damaged 64.1% of the ECV304 cells (p<0.001), and this was confirmed morphologically. Treatment with 1%, 2% or 5% glyserol increased the paracellular permeability of fluorescein isothiocyanate (FITC) -labeled dextran by 4.7%, 18.7% and 29.4% (p<0.05), respectively. In addition, exposure to glycerol at any concentration strongly reduced the expression of occludin, whereas enpression of claudin-1 was affected very slightly. These results suggest that hyperosmotic glycerol would certainly induce the paracellular pathway in vitro and modulate the expression of tight junctional molecules.
本文言語eng
著者キーワードclaudin-1
glycerol
occludin
transcellular permeability
tight junction
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University Graduate School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
49
3-4
開始ページ71
終了ページ79
発行日2002-12
著者版/出版社版出版社版
リポジトリIDA020049000305
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020049000305