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フルテキストURLA050055000301.pdf ( 570.6KB ) 公開日 2010-04-19
タイトルRole of alpha-actinin-4 in the regulation of endocytosis
作成者Hara, Tomohiko
Honda, Kazufumi
Shitashige, Miki
Ono, Masaya
Matsuyama, Hideyasu
Naito, Katsusuke
Hirohashi, Setsuo
Yamada, Tesshi
作成者ヨミハラ, トモヒコ
ホンダ, カズフミ
シタシゲ, ミキ
オノ, マサヤ
マツヤマ, ヒデヤス
ナイトウ, カツスケ
ヒロハシ, セツオ
ヤマダ, テッシ
作成者別表記松山, 豪泰
作成者所属山口大学大学院医学系研究科(医学)
内容記述(抄録等)Alpha-actinin-4, originally identified as an actin-binding protein, has been associated with cell motility and cancer invasion. However, it forms complexes with a diverse array of partner proteins and is speculated to exert several distinct functions based on the characteristics of these proteins. Immunoprecipitation and mass spectrometric analysis reveal that alpha-actinin-4 forms native complexes with several partner proteins in 22RV1 cells, including β/γ-actin, clathrin heavy chain and nonmuscular myosin heavy chain. Clathrin is a coat protein that covers the internalized membrane pit and plays a central role in early endocytosis. We identified other clathrin-related and unrelated cargo proteins, including dynamin, adaptin-γ, β-NAP and p47A, that interact with alpha-actinin-4. Immunofluorescence microscopy revealed that dynamin and clathrin are co-localized with alpha-actinin-4 at the dorsal sites of membrane rufflings, and the transfection of ACTN4 cDNA facilitates the transport of transferrin into the peri-nuclear endosomes. In this review, we report that alpha-actinin-4 is involved in the regulation of endocytosis. Endocytosis enforces termination of signalling evoked by the cell surface receptors and regulates recycling of receptors and ligands. The change of alpha-actinin-4 expression levels may therefore induce aberrations in the intracellular trafficking of various cell surface molecules, such as growth factors and receptors.
本文言語eng
著者キーワードactinin-4
endocytosis
clathrin
proteomics
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University School of Medicine
NII資料タイプ紀要論文
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
55
3-4
開始ページ21
終了ページ26
発行日2008
関連情報URL(IsPartOf)http://ds.cc.yamaguchi-u.ac.jp/~bulletin/index.html
著者版/出版社版出版社版
リポジトリIDA050055000301
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A050055000301