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フルテキストURLA020010000403.pdf ( 629.9KB ) 公開日 2010-04-16
タイトルStudies on the Enzymatic Synthesis of Thiamine by Staphylococcus Aureus
作成者Tsubota, Yoko
Hayashi, Ryoji
作成者ヨミツボタ, ヨウコ
ハヤシ, リョウジ
作成者所属山口大学医学部
内容記述(抄録等)Studies on the enzymatic synthesis of thiamine by Staphylococcus aureus FDA 209-P were carried out and following results were obtained. 1) The active form of pyrimidine was OMP-PP. 2) Free Th as well as its phosphorylated derivatives was sctive as a substrate for the acetone-dried powder of the organisms and the crude enzyme preparation extracted from the powder. 3) Th-P was the most active form of Th for the enzyme preparation with had been purified by the treatment with ammonium sulfate and chromatography on DEAE-cellulose. The activity of free Th for the partially purefied enzyme was negligible. 4) The reaction products from OMP-PP and Th-P of the purified enzyme preparation were TDP and TMP as examined by paper chromatographic and bioautographic method. 5) The activity of the enzyme was increased by the addition of Mn¨ Mg¨or Cd¨. 6) The rhiamine-synthesizing enzyme was comparatively thermostable.
本文言語eng
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University Graduate School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
10
4
開始ページ215
終了ページ225
発行日1963-12
著者版/出版社版出版社版
リポジトリIDA020010000403
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020010000403