| フルテキストURL | フルテキストリンクなし |
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| タイトル | Antioxidant, N-acetyl-L-cysteine inhibits the expression of the collagen α2 (I) promoter in the activated human hepatic stellate cell line in the absence as well as the presence of transforming growth factor-β |
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| 作成者 | Segawa, Makoto
Kayano, Kozo
Sakaguchi, Eiki
Okamoto, Mariko
Sakaida, Isao
Okita, Kiwamu
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| 作成者ヨミ | セガワ, マコト
カヤノ, コウゾウ
サカグチ, エイキ
オカモト, マリコ
サカイダ, イサオ
オキタ, キワム
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| 作成者別表記 | 瀬川, 誠
坂井田, 功
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| 作成者所属 | 山口大学医学部附属病院
山口大学大学院医学系研究科（医学）
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| 内容記述（抄録等） | Although recent studies suggest the inhibitory property of N-acetyl-L-cysteine (NAC) on activation of hepatic stellate cell (HSC), the effects on once-activated HSCs are not well clarified. We investigated the influences of NAC on human-derived once-activated HSC line, LI90 with a focus on the collagen α2 (I) (COL1A2) promoter expression. Plasmid containing whole length of COL1A2 promoter linked to firefly luciferase gene and its various 5′-deletions were transiently transfected to LI90. The luciferase activity was determined with or without 10 mM of NAC in the absence or presence of 1 ng/ml of transforming growth factor (TGF)-β. The effects of NAC on generation of intracellular reactive oxygen species (ROS) in LI90 were also analyzed. As a result, NAC significantly (P<0.05) suppressed the COL1A2 promoter expression in the absence or presence of TGF-β. The expression was much more inhibited when used the deletion containing only AP-1/NF-κB binding sites than that including only three SP-1 binding sites. The ROS production was also comparably inhibited by NAC in both condition. These results indicated NAC suppressed, through its anti-oxidative action, the COL1A2 promoter expression in once-activated HSCs in the absence or presence of TGF-β at least partly by affecting the signal transduction cascade encompassing AP-1/NF-κB activation.
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| 本文言語 | eng
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| 著者キーワード | Liver fibrosis
Hepatic stellate cell
N-acetyl- -cysteine
LI90
COL1A2 promoter
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| 資料タイプ | text |
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| 出版者 | Elsevier
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| NII資料タイプ | 学術雑誌論文 |
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| 査読の有無 | 査読あり |
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| ISSN | 1386-6346
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| NCID | AA11140867
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| 掲載誌名 | Hepatology research : the official journal of the Japan Society of Hepatology |
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| 巻 | 24 |
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| 号 | 3 |
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| 開始ページ | 305 |
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| 終了ページ | 315 |
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| 発行日 | 2002 |
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| DOI | info:doi/10.1016/S1386-6346(02)00089-X |
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| 関連情報URL(IsPartOf) | http://www.sciencedirect.com/science/journal/13866346
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| 著者版/出版社版 | その他 |
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| リポジトリID | 2008010380 |
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| 地域区分 | 山口大学
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| URI | http://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2008010380 |
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