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タイトルAntioxidant, N-acetyl-L-cysteine inhibits the expression of the collagen α2 (I) promoter in the activated human hepatic stellate cell line in the absence as well as the presence of transforming growth factor-β
作成者Segawa, Makoto
Kayano, Kozo
Sakaguchi, Eiki
Okamoto, Mariko
Sakaida, Isao
Okita, Kiwamu
作成者ヨミセガワ, マコト
カヤノ, コウゾウ
サカグチ, エイキ
オカモト, マリコ
サカイダ, イサオ
オキタ, キワム
作成者別表記瀬川, 誠
坂井田, 功
作成者所属山口大学医学部附属病院
山口大学大学院医学系研究科(医学)
内容記述(抄録等)Although recent studies suggest the inhibitory property of N-acetyl-L-cysteine (NAC) on activation of hepatic stellate cell (HSC), the effects on once-activated HSCs are not well clarified. We investigated the influences of NAC on human-derived once-activated HSC line, LI90 with a focus on the collagen α2 (I) (COL1A2) promoter expression. Plasmid containing whole length of COL1A2 promoter linked to firefly luciferase gene and its various 5′-deletions were transiently transfected to LI90. The luciferase activity was determined with or without 10 mM of NAC in the absence or presence of 1 ng/ml of transforming growth factor (TGF)-β. The effects of NAC on generation of intracellular reactive oxygen species (ROS) in LI90 were also analyzed. As a result, NAC significantly (P<0.05) suppressed the COL1A2 promoter expression in the absence or presence of TGF-β. The expression was much more inhibited when used the deletion containing only AP-1/NF-κB binding sites than that including only three SP-1 binding sites. The ROS production was also comparably inhibited by NAC in both condition. These results indicated NAC suppressed, through its anti-oxidative action, the COL1A2 promoter expression in once-activated HSCs in the absence or presence of TGF-β at least partly by affecting the signal transduction cascade encompassing AP-1/NF-κB activation.
本文言語eng
著者キーワードLiver fibrosis
Hepatic stellate cell
N-acetyl- -cysteine
LI90
COL1A2 promoter
資料タイプtext
出版者Elsevier
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN1386-6346
NCIDAA11140867
掲載誌名Hepatology research : the official journal of the Japan Society of Hepatology
24
3
開始ページ305
終了ページ315
発行日2002
DOIinfo:doi/10.1016/S1386-6346(02)00089-X
関連情報URL(IsPartOf)http://www.sciencedirect.com/science/journal/13866346
著者版/出版社版その他
リポジトリID2008010380
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2008010380