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フルテキストURLA020020000102.pdf ( 386.2KB ) 公開日 2010-04-16
タイトルAn Application of Isoelectric Focusing in the Purification of Thiamine-binding Protein of Escherichia coli
作成者Nishimune, Takahiro
Hayashi, Ryoji
作成者ヨミニシムネ, タカヒロ
ハヤシ, リョウジ
作成者所属山口大学医学部
内容記述(抄録等)A partially purified thiamine-binding protein preparation was fractionated by isoelectric fractionation and following results were obtained. 1. The isoelectric fractionation could be used as a method of purification of the binding protein with nearly the same efficiency and recovery as those of conventional purification methods, but it took at least 3-5 days for an expriment. 2. The carrier ampholite covering the pH range of 0.7 (pH 5.5-6.2, 40% concentration) was used in a final concentration of 0.7% with a 440 ml column and was found adequate for the fractionation of 240mg of proteins, which had been liberated from E..coli cells by osmotic shock. 3. The carrier ampholite of the pH range of 0.35 (pH 5.88-6.23) was oreoared and used for the binding protein purification. The final concentration of the ampholite was approx. 0.45% and thiamine-binding activity was recovered in two separate groups of fractions. The recovery in the main activity peak was 51 % with 2.2 times purification in the specific activity and could be used as a purification procedure. 4. The carrier ampholite of narrower pH range(0.35 pH) gave a fractionation product of the specific activity of 8.84 nmole/mg from the sample of 3.96 nmole/mg. The carrier ampholite of 0.7 pH range gave a fractionation product of the specific activity of 1.18 nmole/mg from the sample of 0.61 nmole/mg. Those values indicated that the resolving power of the narrower pH range ampholote was superior to the 0.7 pH range ampholite appreciably.
本文言語eng
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University Graduate School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
20
1-2
開始ページ9
終了ページ16
発行日1973-06
著者版/出版社版出版社版
リポジトリIDA020020000102
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020020000102