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フルテキストURLA020016000209.pdf ( 1.1MB ) 公開日 2010-04-16
タイトルHeat Denaturation Test for Unstable Hemoglobin, with a Note on Its Application to Hemoglobin Survey in Japan
作成者Ueda, Satoshi
Matsuoka, Miyoko
Miyaji, Takaoki
Ueda, Naoki
Aoki, Kenzo
Izawa, Michi
Shibata, Susumu
作成者ヨミウエダ, サトシ
マツオカ, ミヨコ
ミヤジ, タカオキ
ウエダ, ナオキ
アオキ, ケンゾウ
イザワ, ミチ
シバタ, ススム
作成者所属山口大学医学部
内容記述(抄録等) Dacie's heat denaturation test was modified in order to get a practical procedure for detecting unstable hemoglobin in hemolysates. For the purpose of screening a centrifugation method was devised : hemolysate, phosphate buffer solution (0.1 M, pH 7.4), and zinc chloride solution were mixed in equal proportions (0.2 ml each) in a test tube and transferred to a capillary tube (0.3 × 12 cm). The capillary tube was sealed at one end by fusion in a flame of a burner so that the mixture might be contained safely in it, warmed at 50℃ for 60 minutes, and then centrifuged at 3000 rpm (1700 G) for 5 minutes to read the amount of precipitate sedimented at the bottom in terms of the percentage of the total co1umn of the mixture. A reading larger than 3.5 per cent is suggesive of the presence of unstable hemoglobin in hemolysate. Such a hemolysate should be examined by spectrophotometric method to connrm the result. Namely, hemolysate (30 μl) was mixed with warm (55℃) phosphate buffer solution (0.1 M, pH 7.4; 3 ml) and introduced in a cuvette. The absorbance (at 700 mμ) of the mixture was measured continuously in a self-recording spectrophotometer with constant temperature cuvette holder (55℃) for 20 minutes. Hemolysates containing unstable hemoglobin show at least an increment of absorbance two times as large as that obtained with normal hemolysates (usually the normal hemolysates gave an increase in absorbance less than 0.300 at the end of 20 minutes). Abnormal hemoglobins seen in Ube during the period from 1968 to 1969 were examined by these tests, and an unstable hemoglobin (Hb Mie) which was unseparable from normal hemoglobin by electrophoresis was discovered. A short record of the clinical manifestation and laboratory examination of the hemolytic anemia associated with this unstable hemoglobin was presented as an example of the applcation of these methods to hemoglobin survey.
本文言語eng
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University Graduate School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
16
2-4
開始ページ183
終了ページ194
発行日1969-12
著者版/出版社版出版社版
リポジトリIDA020016000209
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020016000209