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フルテキストURLA020027000305.pdf ( 485.6KB ) 公開日 2010-04-19
タイトルThe Periodic Loss of Metabolically Unstable DNA from Newly Replicated DNA of Mouse L Cells
作成者Masuda, Michiyoshi
Yamaoka, Hideki
Isiglo, Kimio
Matsumoto, Takafumi
Takahashi, Manabu
作成者ヨミマスダ, ミチヨシ
ヤマオカ, ヒデキ
イシグロ, キミオ
マツモト, タカフミ
タカハシ, マナブ
作成者所属山口大学医学部
内容記述(抄録等)The kinetic constants for human very low density lipoproteins (VLDL) of membrane-supported lipoprotein lipase (LPL) of the rat were studied. The VLDL substrate (Sf20-400) was obtained from the plasma of healthy human subjects. with normolipidemia and with normal patterns of VLDL apoprotein peptides in isoelectric focusing electrophoretogram. Triglycerides of human VLDL were hydrolyzed by LPL on the vasclar surface of the isolated rat heart. The calculated maximal reaction velocity (Vmax) and the apparent Michaelis constant (Km) were 0.037±0.006μmol min^-1ml^-1 and 0.060±0.004mM (Mean±S.D.), respectively. These values were very close to the corresponding values obtained using rat VLDL as a substrate. This system provides a means to study possible kinetic abnormalities of the triglyceride-rich lipoproteins on the vascular surface of the heart of hyperlipidemic patients.
本文言語eng
著者キーワード^3H-thymidine incorporation
unstable DNA
hydroxyurea
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University Graduate School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
27
3-4
開始ページ117
終了ページ124
発行日1980-12
著者版/出版社版出版社版
リポジトリIDA020027000305
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020027000305