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フルテキストURL2009010011.pdf ( 2.1MB ) 公開日 2010-04-16
タイトルImmunological responses of dogs experimentally infected with Dirofilaria immitis
作成者Song, Kun-Ho
Hayasaki, Mineo
Choliq, Chusnul
Cho, Kyu-Woan
Han, Hong-Ryul
Jeong, Bung-Hyun
Jeon, Moo-Hyung
Park, Bae-Kun
Kim, Duck-Hwan
作成者ヨミSong, Kun-Ho
ハヤサキ, ミネオ
Choliq, Chusnul
Cho, Kyu-Woan
Han, Hong-Ryul
Jeong, Bung-Hyun
Jeon, Moo-Hyung
Park, Bae-Kun
Kim, Duck-Hwan
作成者別表記早崎, 峯夫
作成者所属山口大学農学部
内容記述(抄録等)Three dogs were experimentally infected with Dirofilaria immitis. All dogs were euthanised at 30, 36 and 37 weeks after inoculation of D. immitis for the recovery of adult worms. Three cases accounted to 42.91 % recovery of inoculated worms. Serum samples from dogs experimentally inoculated with D. immitis were analyzed by ELISA and immunoblotting methods. Antibody titers of dogs detected by ELISA peaked between 7 and 14 weeks then decreased between weeks 15 to 24 followed by another increase during weeks 25 to 30 and persisted throughout the remainder of the experiment period. Analysis of adult D. immitis protein stained with Coomassie brilliant blue R-250 indicated separately more than 10 bands, and the major bands were 22, 40, 46, 56, 70, 72 and 89 kDa. Antigenic identification of extracts antigens of adults D. immitis by immunoblotting analysis revealed several bands from pooled sera of patent infection (30 weeks after inoculation). The detected bands were 24, 70, 80 and 110 kDa, 22, 72 and 84 kDa, and 58 and 72 kDa in dogs 1, 2 and 3, respectively. Results of antibody titers reached high levels on the 4th molting stage after inoculation of infective larva (L3), and reinforced previous findings that high molecular weight regions are detected in young animals.
本文言語eng
著者キーワードDirofilaria immitis
experimental infection
ELISA
immunoblotting
資料タイプtext
ファイル形式application/pdf
出版者The Korean Society of Veterinary Science
NII資料タイプ学術雑誌論文
ISSN1229-845X
NCIDAA11489950
掲載誌名Journal of Veterinary Science
3
2
開始ページ109
終了ページ114
発行日2002-06
関連情報URL(IsPartOf)http://www.vetsci.org/
著者版/出版社版出版社版
リポジトリID2009010011
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2009010011